The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. How can i prepare working solution with 1 kb dna ladder supplied by promega. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double. For technical questions about this product, call the invitrogen techlinesm u. Find and purchase 1 kb plus dna ladder products at invitrogen life science technologies. The 1 kb plus dna ladder for safe stains might be used on sybr green i precast agarose gels but might require further dilution and optimization. The 1 kb dna ladder has a number of proprietary plasmids that are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards. A molecularweight size marker, also referred to as a protein ladder, dna ladder, or rna. Try the 1 kb plus dna ladder from neb, a single convenient ladder for 100 bp up to 10 kb. A reference band at 1,500 bp is included for easy orientation. Catalog number size important guidelines 10787018 250 g. Tracking dye does not leave a shadow under uv light. The invitrogen 1kb plus dna ladder is designed for sizing and quantification of double stranded dna on 0.
The key to accurate band analysis is to use the correct dna ladder for your particular application. Do not heat the trackit 1kb plus dna ladder before loading. Is it ok to use dna molecular weight marker in rna. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed. Quickload purple 100 bp dna ladder is a readytoload marker ranging from 1001517 bp. Free sample available, use link below to request free. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments. Download a pdf containing pricing for our full product list. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. It allows sizing and concentration estimate of dna fragments on. A molecularweight size marker in the form of a 1kb dna ladder in the rightmost lane, used in gel electrophoresis. Every readytouse ladder is supplied with the nuclease free loading dye solution, which ensures optimal migration and quantification of your dna probes. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.
This product is available in quickload purple format enjoy the convenience of a readytoload format. General description sigmas directload 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Is it ok to use dna molecular weight marker in rna electrophoresis. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Use the invitrogen dna ladder selection table below to help you choose the dna ladder you need invitrogen. Gel loading buffer timing varies depending on amplicon length selection guide pcr enzymes and master mixes go online to view related products. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. A number of proprietary plasmids are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. How can i prepare working solution with 1 kb dna ladder.
Generuler 1 kb plus dna ladder thermo fisher scientific. The dna ladder consists of proprietary plasmids which are. Nucleic acid ladder is used as a size standard in agarose gel electrophoresis. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Dna marker bp 1 kb is designed for use as molecular weight standard for agarose gel electrophoresis features.
This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. B qrtpcr was performed for reep5 and reep6 mrna in a549 cells infected with lentivirus. So im wondering if maybe the dna ladder ran differently in the gel compared to the rna samples. Dna marker bp 1 kb blue is designed for use as molecular weight standard for agarose gel electrophoresis availibility of sample size may be limited. Gel conditions are 1% agarose, 3 voltcm, and ethidium bromide stain. The bands of the ladder each contain from 1 to 12 repeats of a 1018.
The 1 kb plus dna ladder ranges from 100bp 10kb and and is supplied with a free vial of gel loading dye, purple 6x, no sds. Nebs quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands. Sm, size markers 1 kb plus dna ladder from invitrogen. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. Directload 1 kb dna ladder readytouse marker for dna. This marker is ideal for the size determination of digested dna. Find and order marker and products like excelband 1kb plus 0. Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas. Highranger 1kb dna ladder 300bp 0bp size determination of digested dna. The accessory proteins reep5 and reep6 refine cxcr1. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp.
The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. The 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments ranging in size from 100 bp to 15,000 bp. The 1 kb plus dna ladder for safe stains is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. The ladder is premixed with gel load buffer color dyes with varying mobility on an. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb.
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